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高敏型大鼠C反應(yīng)蛋白(CRP)酶聯(lián)免疫吸附檢測試劑盒

一鍵復(fù)制產(chǎn)品信息

ELK9996HS

規(guī)格：	價格：
48T	￥1680.00
96T	￥2400.00
96T*5	￥10200.00

下載說明 ①

Overview

Product name:	High Sensitivity Rat CRP(C Reactive Protein) ELISA Kit
Reactivity:	Rat
Alternative Names:	C-RP; PTX1; Pentraxin-Related; Pentraxin 1
Assay Type:	Sandwich
Sensitivity:	1.475 pg/mL
Standard:	500 pg/mL
Detection Range:	7.81-500 pg/mL
Sample Type:	Serum, plasma
Assay Length:	3.5h
Research Area:	Tumor immunity;Infection immunity;Cardiovascular biology;Rheumatology;
Uniprot ID:	P48199
Test principle:	The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Rat CRP. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Rat CRP. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Rat CRP, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Rat CRP in the samples is then determined by comparing the OD of the samples to the standard curve.

標(biāo)準曲線

Concentration (pg/mL)	OD	Corrected OD
500.00	2.389	2.307
250.00	1.593	1.511
125.00	1.213	1.131
62.50	0.882	0.800
31.25	0.585	0.503
15.63	0.305	0.223
7.82	0.232	0.150
0.00	0.082	0.000

精密度

Intra-assay Precision (Precision within an assay)：CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision.

Inter-assay Precision (Precision between assays)：CV%<10%

Three samples of known concentration were tested in forty separate assays to assess inter-assay precision.

回收率

Matrices listed below were spiked with certain level of recombinant CRP and the recovery rates were calculated by comparing the measured value to the expected amount of CRP in samples.

Matrix	Recovery range	Average
serum(n=5)	86-94%	90%
EDTA plasma(n=5)	77-84%	81%
Heparin plasma(n=5)	84-90%	87%

線性

The linearity of the kit was assayed by testing samples spiked with appropriate concentration of CRP and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.