91自拍网在线播放,亚洲国产精品无码久久久不卡,四虎永久地址www成人久久

中文字幕在线乱码日本-亚洲熟女综合色一区二区三区四区-欧美一区二区三区中文字幕-日韩在线观看视频91-国产日韩精品一区二区三区在线-欧美精品日韩在线亚洲另类-国内精品久久久久久一区二区-曰本熟女一区二区三区-成人动漫一二三区在线观看

產(chǎn)品中心

高敏型小鼠生長(zhǎng)調(diào)節(jié)致癌基因β(GROb)酶聯(lián)免疫吸附檢測(cè)試劑盒

一鍵復(fù)制產(chǎn)品信息

ELK1383HS

規(guī)格：	價(jià)格：
48T	￥1680.00
96T	￥2400.00
96T*5	￥10200.00

下載說(shuō)明 ①

Overview

Product name:	High Sensitivity Mouse GROb(Growth Regulated Oncogene Beta) ELISA Kit
Reactivity:	Mouse
Alternative Names:	CXCL2; SCYB2; MIP2; GRO2; MIP2a; MGSAb; CINC2a; HSF; Macrophage inflammatory protein 2-alpha; Hematopoietic synergistic factor; Chemokine(C-X-C-Motif)ligand 2
Assay Type:	Sandwich
Sensitivity:	0.94 pg/mL
Standard:	200 pg/mL
Detection Range:	3.13-200 pg/mL
Sample Type:	Serum, plasma
Assay Length:	3.5h
Research Area:	Cytokine;Tumor immunity;Infection immunity;
Test principle:	The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Mouse GROb. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Mouse GROb. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Mouse GROb, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Mouse GROb in the samples is then determined by comparing the OD of the samples to the standard curve.

標(biāo)準(zhǔn)曲線

Concentration (pg/mL)	OD	Corrected OD
200.00	2.302	2.209
100.00	1.736	1.643
50.00	1.226	1.133
25.00	0.861	0.768
12.50	0.595	0.502
6.25	0.326	0.233
3.13	0.226	0.133
0.00	0.093	0.000

精密度

Intra-assay Precision (Precision within an assay)：CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision.

Inter-assay Precision (Precision between assays)：CV%<10%

Three samples of known concentration were tested in forty separate assays to assess inter-assay precision.

回收率

Matrices listed below were spiked with certain level of recombinant GROb and the recovery rates were calculated by comparing the measured value to the expected amount of GROb in samples.

Matrix	Recovery range	Average
serum(n=5)	82-91%	87%
EDTA plasma(n=5)	79-89%	84%
Heparin plasma(n=5)	81-87%	84%

線性

The linearity of the kit was assayed by testing samples spiked with appropriate concentration of GROb and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.