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人中段心房利鈉肽(MR-ProANP)微量上樣酶聯(lián)免疫吸附檢測(cè)試劑盒

一鍵復(fù)制產(chǎn)品信息

ELK0872MS

規(guī)格：	價(jià)格：
48T	￥1820.00
96T	￥2600.00
96T*5	￥11050.00

下載說(shuō)明 ①

Overview

Product name:	Human MR-proANP(midregional pro-atrial natriuretic peptide) Microsample ELISA Kit
Reactivity:	Human
Alternative Names:	MR-proANP
Assay Type:	Sandwich
Sensitivity:	0.05 ng/mL
Standard:	10 ng/mL
Detection Range:	0.16-10 ng/mL
Sample Type:	Serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids
Assay Length:	3.5h
Research Area:	Endocrinology;Cardiovascular biology;
Test principle:	The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Human MR-proANP. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Human MR-proANP. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Human MR-proANP, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Human MR-proANP in the samples is then determined by comparing the OD of the samples to the standard curve.

標(biāo)準(zhǔn)曲線

Concentration (ng/mL)	OD	Corrected OD
10.00	2.151	2.054
5.00	1.742	1.645
2.50	1.209	1.112
1.25	0.888	0.791
0.63	0.532	0.435
0.31	0.342	0.245
0.16	0.209	0.112
0.00	0.097	0.000

精密度

Intra-assay Precision (Precision within an assay)：CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision.

Inter-assay Precision (Precision between assays)：CV%<10%

Three samples of known concentration were tested in forty separate assays to assess inter-assay precision.

回收率

Matrices listed below were spiked with certain level of recombinant MR-proANP and the recovery rates were calculated by comparing the measured value to the expected amount of MR-proANP in samples.

Matrix	Recovery range	Average
serum(n=5)	82-118%	100%
EDTA plasma(n=5)	91-118%	105%
Heparin plasma(n=5)	83-98%	91%

線性

The linearity of the kit was assayed by testing samples spiked with appropriate concentration of MR-proANP and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.