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人細(xì)胞角蛋白18-M30(CK 18-M30)微量上樣酶聯(lián)免疫吸附檢測試劑盒

一鍵復(fù)制產(chǎn)品信息

ELK0871MS

規(guī)格：	價格：
48T	￥1960.00
96T	￥2800.00
96T*5	￥11900.00

下載說明 ①

Overview

Product name:	Human CK 18-M30 (Cytokeratin 18-M30) Microsample ELISA Kit
Reactivity:	Human
Alternative Names:	K18-M30; CK-18-M30; CYK18-M30
Assay Type:	Sandwich
Sensitivity:	7 μIU/mL
Standard:	1000 μIU/mL
Detection Range:	15.63-1000 μIU/mL
Sample Type:	Serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids
Assay Length:	3.5h
Research Area:	Cancer of the liver; Viral hepatitis
Test principle:	The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Human CK 18-M30. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Human CK 18-M30. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Human CK 18-M30, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Human CK 18-M30 in the samples is then determined by comparing the OD of the samples to the standard curve.

標(biāo)準(zhǔn)曲線

Concentration (μIU/mL)	OD	Corrected OD
1000.00	1.964	1.875
500.00	1.477	1.388
250.00	1.116	1.027
125.00	0.668	0.579
62.50	0.490	0.401
31.25	0.309	0.220
15.63	0.196	0.107
0.00	0.089	0.000

精密度

Intra-assay Precision (Precision within an assay)：CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision.

Inter-assay Precision (Precision between assays)：CV%<10%

Three samples of known concentration were tested in forty separate assays to assess inter-assay precision.

回收率

Matrices listed below were spiked with certain level of recombinant CK 18-M30 and the recovery rates were calculated by comparing the measured value to the expected amount of CK 18-M30 in samples.

Matrix	Recovery range	Average
serum(n=5)	87-102%	95%
EDTA plasma(n=5)	85-109%	97%
Heparin plasma(n=5)	90-103%	97%

線性

The linearity of the kit was assayed by testing samples spiked with appropriate concentration of CK 18-M30 and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.