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大鼠高敏型心肌肌鈣蛋白T(hs-cTnT)微量上樣酶聯(lián)免疫吸附檢測試劑盒

一鍵復(fù)制產(chǎn)品信息

ELK0844MS

規(guī)格：	價格：
48T	￥1960.00
96T	￥2800.00
96T*5	￥11900.00

下載說明 ①

Overview

Product name:	Rat hs-cTnT(High Sensitivity Cardiac Troponin T) Microsample ELISA Kit
Reactivity:	Rat
Alternative Names:	High Sensitivity Cardiac Troponin T
Assay Type:	Sandwich
Sensitivity:	5.3 pg/mL
Standard:	1000 pg/mL
Detection Range:	15.63-1000 pg/mL
Sample Type:	serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids
Assay Length:	3.5h
Research Area:	Cardiovascular biology
Test principle:	The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Rat hs-cTnT. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Rat hs-cTnT. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Rat hs-cTnT, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Rat hs-cTnT in the samples is then determined by comparing the OD of the samples to the standard curve.

標(biāo)準(zhǔn)曲線

Concentration (pg/mL)	OD	Corrected OD
1000.00	2.096	2.014
500.00	1.670	1.588
250.00	1.114	1.032
125.00	0.830	0.748
62.50	0.524	0.442
31.25	0.322	0.240
15.63	0.259	0.177
0.00	0.082	0.000

精密度

Intra-assay Precision (Precision within an assay)：CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision.

Inter-assay Precision (Precision between assays)：CV%<10%

Three samples of known concentration were tested in forty separate assays to assess inter-assay precision.

回收率

Matrices listed below were spiked with certain level of recombinant hs-cTnT and the recovery rates were calculated by comparing the measured value to the expected amount of hs-cTnT in samples.

Matrix	Recovery range	Average
serum(n=5)	92-111%	102%
EDTA plasma(n=5)	82-91%	87%
Heparin plasma(n=5)	92-118%	105%

線性

The linearity of the kit was assayed by testing samples spiked with appropriate concentration of hs-cTnT and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.