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人涎液化糖鏈抗原6(KL-6)微量上樣酶聯(lián)免疫吸附檢測試劑盒

一鍵復制產品信息

ELK0179MS

規(guī)格：	價格：
48T	￥1960.00
96T	￥2800.00
96T*5	￥11900.00

下載說明 ①

Overview

Product name:	Human KL-6(Krebs Von den Lungen-6) Microsample ELISA Kit
Reactivity:	Human
Alternative Names:	KL-6
Assay Type:	Sandwich
Sensitivity:	14.25 U/mL
Standard:	2000 U/mL
Detection Range:	31.25-2000 U/mL
Sample Type:	serum, plasma, tissue homogenates and other biological fluids
Assay Length:	3.5h
Research Area:	Interstitial lung disease
Test principle:	The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Human KL-6. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Human KL-6. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Human KL-6, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Human KL-6 in the samples is then determined by comparing the OD of the samples to the standard curve.

標準曲線

Concentration (U/mL)	OD	Corrected OD
2000.00	1.906	1.810
1000.00	1.516	1.420
500.00	1.188	1.092
250.00	0.786	0.690
125.00	0.506	0.410
62.50	0.378	0.282
31.25	0.228	0.132
0.00	0.096	0.000

精密度

Intra-assay Precision (Precision within an assay)：CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision.

Inter-assay Precision (Precision between assays)：CV%<10%

Three samples of known concentration were tested in forty separate assays to assess inter-assay precision.

回收率

Matrices listed below were spiked with certain level of recombinant KL-6 and the recovery rates were calculated by comparing the measured value to the expected amount of KL-6 in samples.

Matrix	Recovery range	Average
serum(n=5)	93-109%	101%
EDTA plasma(n=5)	87-119%	103%
Heparin plasma(n=5)	92-109%	101%

線性

The linearity of the kit was assayed by testing samples spiked with appropriate concentration of KL-6 and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.